Phenomic Analysis of a Collection of T-DNA Insertion Lines in Rice



In an effort to contribute to the International Functional Genomics Consortium, Dr E. Guiderdoni’s lab (Cirad, Montpellier) developed a library of T-DNA insertion lines (Sallaud et al 2004). These lines were characterized at CIAT between 2003 and 2008 through an extensive phenomics study at the field level (Lorieux et al 2012). A total of 28,000 lines were scored for a series of standard traits defined by the Trait Ontology Consortium. The acquired data, together with the flanking sequence tags (FSTs) of the T-DNA and Tos17 insertions, provide a powerful tool for reverse genetic analyses. The Paddy Genes Book database was developed and is available upon request to the author. An online version, Oryza Tag Line, is also available (Larmande et al 2008). Seeds of T-DNA lines should be ordered from Dr Guiderdoni at Cirad.